A vasoactive peptide, endothelin-3, is produced by and specifically binds to primary astrocytes.


Journal Article

Primary rat astrocytes were found by immunohistochemistry to display positive staining for endothelin-3, located predominantly in the perinuclear area. The ability of these cells to produce and release endothelin-3 was confirmed by a combination of reverse-phase HPLC and radioimmunoassay, specific for endothelin-3, which demonstrated immunoreactive peptide in cellular extracts and astrocyte-conditioned medium. In addition, astrocytes were shown to possess a single class of binding sites for endothelin with comparable high affinity for endothelin-1, -2 and -3. These results suggest that astrocytes, by virtue of their ability to produce and secrete endothelin-3, serve as a potential extravascular source of intracerebral vasoregulation capable of influencing regional cerebral blood flow.

Full Text

Duke Authors

Cited Authors

  • Ehrenreich, H; Kehrl, JH; Anderson, RW; Rieckmann, P; Vitkovic, L; Coligan, JE; Fauci, AS

Published Date

  • January 4, 1991

Published In

Volume / Issue

  • 538 / 1

Start / End Page

  • 54 - 58

PubMed ID

  • 1850319

Pubmed Central ID

  • 1850319

International Standard Serial Number (ISSN)

  • 0006-8993

Digital Object Identifier (DOI)

  • 10.1016/0006-8993(91)90375-6


  • eng

Conference Location

  • Netherlands