The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex.
Journal Article (Journal Article)
Substitution of one non-bridging oxygen in a natural phosphodiester internucleotide linkage with a borano (-BH3) group results in a chiral phosphorus center in boranophosphate. UV thermal melting profiles were recorded for DNA duplexes formed between a DNA 9-mer with either an Sp or a Rp boranophosphate linkage in the middle and the complementary DNA 9-mer, as well as for their unmodified parent duplex. The thermal stability of the DNA duplexes was in the order of normal > Sp borano > Rp borano. CD spectra of all three duplexes exhibited typical B-DNA profile, which closely resembled each other.
- Wang, JX; Sergueev, DS; Shaw, BR
- January 2005
Volume / Issue
- 24 / 5-7
Start / End Page
- 951 - 955
Electronic International Standard Serial Number (EISSN)
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)