The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex.

Journal Article

Substitution of one non-bridging oxygen in a natural phosphodiester internucleotide linkage with a borano (-BH3) group results in a chiral phosphorus center in boranophosphate. UV thermal melting profiles were recorded for DNA duplexes formed between a DNA 9-mer with either an Sp or a Rp boranophosphate linkage in the middle and the complementary DNA 9-mer, as well as for their unmodified parent duplex. The thermal stability of the DNA duplexes was in the order of normal > Sp borano > Rp borano. CD spectra of all three duplexes exhibited typical B-DNA profile, which closely resembled each other.

Full Text

Duke Authors

Cited Authors

  • Wang, JX; Sergueev, DS; Shaw, BR

Published Date

  • 2005

Published In

Volume / Issue

  • 24 / 5-7

Start / End Page

  • 951 - 955

PubMed ID

  • 16248070

International Standard Serial Number (ISSN)

  • 1525-7770

Language

  • eng

Conference Location

  • United States