Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms.
Publication
, Journal Article
Dhungana, S; Anderson, DS; Mietzner, TA; Crumbliss, AL
Published in: Journal of inorganic biochemistry
November 2004
Two synergistic anions, p-nitrophenyl phosphate ester (NPP) and SO(4)(2-), were found to form new stable assemblies with Fe(3+) and a bacterial transferrin, FbpA (FbpA=ferric binding protein). Fe(3+)FbpA-SO(4) undergoes rapid anion exchange in the presence of NPP to form Fe(3+)FbpA-NPP. Formation of Fe(3+)FbpA-NPP was found to accelerate the rate of hydrolysis of the bound phosphate ester (k(hyd)=1.6 x 10(-6) s(-1) at 25 degrees C and pH 6.5) by >10(3) fold over the uncatalyzed reaction. These findings suggest a dual function for FbpA in vivo: transport of Fe(3+) across the periplasmic space to the inner membrane in certain gram-negative bacteria and hydrolysis of periplasmic polyphosphates.
Duke Scholars
Published In
Journal of inorganic biochemistry
DOI
EISSN
1873-3344
ISSN
0162-0134
Publication Date
November 2004
Volume
98
Issue
11
Start / End Page
1975 / 1977
Related Subject Headings
- Transferrin
- Sulfates
- Spectrophotometry
- Organophosphates
- Iron
- Inorganic & Nuclear Chemistry
- Catalysis
- Carrier Proteins
- Anions
- 3402 Inorganic chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Dhungana, S., Anderson, D. S., Mietzner, T. A., & Crumbliss, A. L. (2004). Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms. Journal of Inorganic Biochemistry, 98(11), 1975–1977. https://doi.org/10.1016/j.jinorgbio.2004.08.004
Dhungana, Suraj, Damon S. Anderson, Timothy A. Mietzner, and Alvin L. Crumbliss. “Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms.” Journal of Inorganic Biochemistry 98, no. 11 (November 2004): 1975–77. https://doi.org/10.1016/j.jinorgbio.2004.08.004.
Dhungana S, Anderson DS, Mietzner TA, Crumbliss AL. Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms. Journal of inorganic biochemistry. 2004 Nov;98(11):1975–7.
Dhungana, Suraj, et al. “Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms.” Journal of Inorganic Biochemistry, vol. 98, no. 11, Nov. 2004, pp. 1975–77. Epmc, doi:10.1016/j.jinorgbio.2004.08.004.
Dhungana S, Anderson DS, Mietzner TA, Crumbliss AL. Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms. Journal of inorganic biochemistry. 2004 Nov;98(11):1975–1977.
Published In
Journal of inorganic biochemistry
DOI
EISSN
1873-3344
ISSN
0162-0134
Publication Date
November 2004
Volume
98
Issue
11
Start / End Page
1975 / 1977
Related Subject Headings
- Transferrin
- Sulfates
- Spectrophotometry
- Organophosphates
- Iron
- Inorganic & Nuclear Chemistry
- Catalysis
- Carrier Proteins
- Anions
- 3402 Inorganic chemistry