The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro.
Publication
, Journal Article
Zehring, WA; Greenleaf, AL
Published in: J Biol Chem
May 25, 1990
We show that the mammalian transcription Sp1 stimulates accurate transcription in a partially fractionated RNA polymerase II-dependent system from Drosophila cultured cells. Moreover, the extent of stimulation is equal for intact RNA polymerase II (polymerase IIA) and polymerase lacking the unique carboxyl-terminal domain of the largest subunit (polymerase IIB). We conclude that in this system Sp1 interacts with a component of the transcription machinery, other than the carboxyl-terminal domain, which is preserved between mammals and insects.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
May 25, 1990
Volume
265
Issue
15
Start / End Page
8351 / 8353
Location
United States
Related Subject Headings
- Transcription, Genetic
- Transcription Factors
- Sp1 Transcription Factor
- Repetitive Sequences, Nucleic Acid
- RNA Polymerase II
- Promoter Regions, Genetic
- Oligonucleotide Probes
- Molecular Sequence Data
- Heat-Shock Proteins
- Drosophila
Citation
APA
Chicago
ICMJE
MLA
NLM
Zehring, W. A., & Greenleaf, A. L. (1990). The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro. J Biol Chem, 265(15), 8351–8353.
Zehring, W. A., and A. L. Greenleaf. “The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro.” J Biol Chem 265, no. 15 (May 25, 1990): 8351–53.
Zehring WA, Greenleaf AL. The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro. J Biol Chem. 1990 May 25;265(15):8351–3.
Zehring, W. A., and A. L. Greenleaf. “The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro.” J Biol Chem, vol. 265, no. 15, May 1990, pp. 8351–53.
Zehring WA, Greenleaf AL. The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro. J Biol Chem. 1990 May 25;265(15):8351–8353.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
May 25, 1990
Volume
265
Issue
15
Start / End Page
8351 / 8353
Location
United States
Related Subject Headings
- Transcription, Genetic
- Transcription Factors
- Sp1 Transcription Factor
- Repetitive Sequences, Nucleic Acid
- RNA Polymerase II
- Promoter Regions, Genetic
- Oligonucleotide Probes
- Molecular Sequence Data
- Heat-Shock Proteins
- Drosophila