Molecular cloning of sequences from a Drosophila RNA polymerase II locus by P element transposon tagging.

Journal Article (Journal Article)

We have identified a lethal mutation in the D. melanogaster RNA polymerase II locus, RpIIC4, caused by insertion of a transposable element associated with the phenomenon of hybrid dysgenesis (P element). Using previously cloned P element sequences as a hybridization probe we have isolated a hybrid lambda phage clone carrying a 10 kb genomic DNA fragment containing a 1.3 kb P element insert and flanking sequences from the RpII locus. The non-P sequences in this clone (lambda DmRpII-1) hybridize to polytene chromosome band region 10C, the cytogenetic location of RpIIC4, and revertants which lose the lethal RNA polymerase II mutation also lose P element sequences from the locus. We have generated several additional P element insertions into the locus and shown that they can occur at two or more different sites. These experiments illustrate that mutagenesis by P element insertion and use of cloned P DNA to retrieve the DNA sequences into which insertion has occurred may be a general method for cloning genetically defined loci in Drosophila.

Full Text

Duke Authors

Cited Authors

  • Searles, LL; Jokerst, RS; Bingham, PM; Voelker, RA; Greenleaf, AL

Published Date

  • December 1, 1982

Published In

Volume / Issue

  • 31 / 3 Pt 2

Start / End Page

  • 585 - 592

PubMed ID

  • 6297774

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(82)90314-2


  • eng

Conference Location

  • United States