Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila.

Journal Article

We have characterized RpII215, the gene encoding the largest subunit of RNA polymerase II in Drosophila melanogaster. DNA sequencing and nuclease S1 analyses provided the primary structure of this gene, its 7 kb RNA and 215 kDa protein products. The amino-terminal 80% of the subunit harbors regions with strong homology to the beta' subunit of Escherichia coli RNA polymerase and to the largest subunits of other eukaryotic RNA polymerases. The carboxyl-terminal 20% of the subunit is composed of multiple repeats of a seven amino acid consensus sequence, Tyr-Ser-Pro-Thr-Ser-Pro-Ser. The homology domains, as well as the unique carboxyl-terminal structure, are considered in the light of current knowledge of RNA polymerase II and the properties of its largest subunit. Additionally, germline transformation demonstrated that a 9.4 kb genomic DNA segment containing the alpha-amanitin-resistant allele, RpII215C4, includes all sequences required to produce amanitin-resistant transformants.

Full Text

Duke Authors

Cited Authors

  • Jokerst, RS; Weeks, JR; Zehring, WA; Greenleaf, AL

Published Date

  • January 1989

Published In

Volume / Issue

  • 215 / 2

Start / End Page

  • 266 - 275

PubMed ID

  • 2496296

International Standard Serial Number (ISSN)

  • 0026-8925

Language

  • eng

Conference Location

  • Germany