Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.
Published
Journal Article
A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.
Full Text
Duke Authors
Cited Authors
- Greenleaf, AL
Published Date
- November 25, 1983
Published In
Volume / Issue
- 258 / 22
Start / End Page
- 13403 - 13406
PubMed ID
- 6417125
Pubmed Central ID
- 6417125
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States