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Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.

Publication ,  Journal Article
Greenleaf, AL
Published in: J Biol Chem
November 25, 1983

A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 25, 1983

Volume

258

Issue

22

Start / End Page

13403 / 13406

Location

United States

Related Subject Headings

  • RNA Polymerase II
  • Protein Multimerization
  • Plasmids
  • Mutation
  • Molecular Weight
  • Macromolecular Substances
  • Genes
  • Drosophila melanogaster
  • Biochemistry & Molecular Biology
  • Animals
 

Citation

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ICMJE
MLA
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Greenleaf, A. L. (1983). Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem, 258(22), 13403–13406.
Greenleaf, A. L. “Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.J Biol Chem 258, no. 22 (November 25, 1983): 13403–6.
Greenleaf AL. Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem. 1983 Nov 25;258(22):13403–6.
Greenleaf, A. L. “Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.J Biol Chem, vol. 258, no. 22, Nov. 1983, pp. 13403–06.
Greenleaf AL. Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem. 1983 Nov 25;258(22):13403–13406.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 25, 1983

Volume

258

Issue

22

Start / End Page

13403 / 13406

Location

United States

Related Subject Headings

  • RNA Polymerase II
  • Protein Multimerization
  • Plasmids
  • Mutation
  • Molecular Weight
  • Macromolecular Substances
  • Genes
  • Drosophila melanogaster
  • Biochemistry & Molecular Biology
  • Animals