Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.
Publication
, Journal Article
Greenleaf, AL
Published in: J Biol Chem
November 25, 1983
A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
November 25, 1983
Volume
258
Issue
22
Start / End Page
13403 / 13406
Location
United States
Related Subject Headings
- RNA Polymerase II
- Protein Multimerization
- Plasmids
- Mutation
- Molecular Weight
- Macromolecular Substances
- Genes
- Drosophila melanogaster
- Biochemistry & Molecular Biology
- Animals
Citation
APA
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ICMJE
MLA
NLM
Greenleaf, A. L. (1983). Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem, 258(22), 13403–13406.
Greenleaf, A. L. “Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.” J Biol Chem 258, no. 22 (November 25, 1983): 13403–6.
Greenleaf AL. Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem. 1983 Nov 25;258(22):13403–6.
Greenleaf, A. L. “Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.” J Biol Chem, vol. 258, no. 22, Nov. 1983, pp. 13403–06.
Greenleaf AL. Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem. 1983 Nov 25;258(22):13403–13406.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
November 25, 1983
Volume
258
Issue
22
Start / End Page
13403 / 13406
Location
United States
Related Subject Headings
- RNA Polymerase II
- Protein Multimerization
- Plasmids
- Mutation
- Molecular Weight
- Macromolecular Substances
- Genes
- Drosophila melanogaster
- Biochemistry & Molecular Biology
- Animals