Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.

Journal Article

A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.

Full Text

Duke Authors

Cited Authors

  • Greenleaf, AL

Published Date

  • November 25, 1983

Published In

Volume / Issue

  • 258 / 22

Start / End Page

  • 13403 - 13406

PubMed ID

  • 6417125

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States