Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II.

Journal Article (Journal Article)

Following EMS mutagenesis we recovered a mutant of D. melanogaster that grows at concentrations of alpha-amanitin lethal to wild-type. To our knowledge this mutant represents the first example of an amanitin-resistant eucaryotic organism. The amanitin resistance of the mutant (AmaC4) is due to an alteration in its DNA-dependent RNA polymerase II, which is approximately 250 times less sensitive to inhibition by amanitin than the wild-type polymerase II whether tested in nuclei, in partially-fractionated extracts or as a highly purified enzyme. While the wild-type enzyme activity is inhibited 50% by 2.1 x 10(-8) M alpha-amanitin, inhibition of 50% of the AmaC4 RNA polymerase II activity requires a toxin concentration of 5.6 x 10(-6) M. The mutation responsible for the amanitin resistance of AmaC4 is on the X chromosome near the vermillion locus.

Full Text

Duke Authors

Cited Authors

  • Greenleaf, AL; Borsett, LM; Jiamachello, PF; Coulter, DE

Published Date

  • November 1, 1979

Published In

Volume / Issue

  • 18 / 3

Start / End Page

  • 613 - 622

PubMed ID

  • 117900

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(79)90116-8


  • eng

Conference Location

  • United States