Skip to main content
Journal cover image

Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II.

Publication ,  Journal Article
Biggs, J; Searles, LL; Greenleaf, AL
Published in: Cell
September 1985

The Drosophila melanogaster RpII215 locus encodes the largest subunit of RNA polymerase II. We have now mapped the 7 kb transcript of the locus and have determined that it contains four exons and three introns. By sequencing 2582 nucleotides from the promoter-proximal end of the RpII215 locus, we have precisely mapped the start site of transcription and the splice sites of the first intron. Segments of the amino acid sequence predicted by the only long open reading frame of the RpII215 gene transcript display striking homology with corresponding segments of the beta subunit of E. coli RNA polymerase.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1985

Volume

42

Issue

2

Start / End Page

611 / 621

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Single-Strand Specific DNA and RNA Endonucleases
  • RNA, Messenger
  • RNA Splicing
  • RNA Polymerase II
  • Promoter Regions, Genetic
  • Genes
  • Escherichia coli
  • Endonucleases
  • Drosophila melanogaster
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Biggs, J., Searles, L. L., & Greenleaf, A. L. (1985). Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II. Cell, 42(2), 611–621. https://doi.org/10.1016/0092-8674(85)90118-7
Biggs, J., L. L. Searles, and A. L. Greenleaf. “Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II.Cell 42, no. 2 (September 1985): 611–21. https://doi.org/10.1016/0092-8674(85)90118-7.
Biggs, J., et al. “Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II.Cell, vol. 42, no. 2, Sept. 1985, pp. 611–21. Pubmed, doi:10.1016/0092-8674(85)90118-7.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1985

Volume

42

Issue

2

Start / End Page

611 / 621

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Single-Strand Specific DNA and RNA Endonucleases
  • RNA, Messenger
  • RNA Splicing
  • RNA Polymerase II
  • Promoter Regions, Genetic
  • Genes
  • Escherichia coli
  • Endonucleases
  • Drosophila melanogaster