Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.

Published

Journal Article

Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.

Full Text

Duke Authors

Cited Authors

  • Ingles, CJ; Biggs, J; Wong, JK; Weeks, JR; Greenleaf, AL

Published Date

  • June 1, 1983

Published In

Volume / Issue

  • 80 / 11

Start / End Page

  • 3396 - 3400

PubMed ID

  • 6407013

Pubmed Central ID

  • 6407013

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.80.11.3396

Language

  • eng

Conference Location

  • United States