Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.

Journal Article

Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.

Full Text

Duke Authors

Cited Authors

  • Ingles, CJ; Biggs, J; Wong, JK; Weeks, JR; Greenleaf, AL

Published Date

  • June 1, 1983

Published In

Volume / Issue

  • 80 / 11

Start / End Page

  • 3396 - 3400

PubMed ID

  • 6407013

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States