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Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II.

Publication ,  Journal Article
Rodriguez, CR; Cho, EJ; Keogh, MC; Moore, CL; Greenleaf, AL; Buratowski, S
Published in: Mol Cell Biol
January 2000

The cotranscriptional placement of the 7-methylguanosine cap on pre-mRNA is mediated by recruitment of capping enzyme to the phosphorylated carboxy-terminal domain (CTD) of RNA polymerase II. Immunoblotting suggests that the capping enzyme guanylyltransferase (Ceg1) is stabilized in vivo by its interaction with the CTD and that serine 5, the major site of phosphorylation within the CTD heptamer consensus YSPTSPS, is particularly important. We sought to identify the CTD kinase responsible for capping enzyme targeting. The candidate kinases Kin28-Ccl1, CTDK1, and Srb10-Srb11 can each phosphorylate a glutathione S-transferase-CTD fusion protein such that capping enzyme can bind in vitro. However, kin28 mutant alleles cause reduced Ceg1 levels in vivo and exhibit genetic interactions with a mutant ceg1 allele, while srb10 or ctk1 deletions do not. Therefore, only the TFIIH-associated CTD kinase Kin28 appears necessary for proper capping enzyme targeting in vivo. Interestingly, levels of the polyadenylation factor Pta1 are also reduced in kin28 mutants, while several other polyadenylation factors remain stable. Pta1 in yeast extracts binds specifically to the phosphorylated CTD, suggesting that this interaction may mediate coupling of polyadenylation and transcription.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

January 2000

Volume

20

Issue

1

Start / End Page

104 / 112

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • RNA, Messenger
  • RNA, Fungal
  • RNA Precursors
  • RNA Polymerase II
  • Protein Serine-Threonine Kinases
  • Mutation
  • Developmental Biology
 

Citation

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Rodriguez, C. R., Cho, E. J., Keogh, M. C., Moore, C. L., Greenleaf, A. L., & Buratowski, S. (2000). Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol, 20(1), 104–112. https://doi.org/10.1128/MCB.20.1.104-112.2000
Rodriguez, C. R., E. J. Cho, M. C. Keogh, C. L. Moore, A. L. Greenleaf, and S. Buratowski. “Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II.Mol Cell Biol 20, no. 1 (January 2000): 104–12. https://doi.org/10.1128/MCB.20.1.104-112.2000.
Rodriguez CR, Cho EJ, Keogh MC, Moore CL, Greenleaf AL, Buratowski S. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol. 2000 Jan;20(1):104–12.
Rodriguez, C. R., et al. “Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II.Mol Cell Biol, vol. 20, no. 1, Jan. 2000, pp. 104–12. Pubmed, doi:10.1128/MCB.20.1.104-112.2000.
Rodriguez CR, Cho EJ, Keogh MC, Moore CL, Greenleaf AL, Buratowski S. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol. 2000 Jan;20(1):104–112.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

January 2000

Volume

20

Issue

1

Start / End Page

104 / 112

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • RNA, Messenger
  • RNA, Fungal
  • RNA Precursors
  • RNA Polymerase II
  • Protein Serine-Threonine Kinases
  • Mutation
  • Developmental Biology