Sites of P element insertion and structures of P element deletions in the 5' region of Drosophila melanogaster RpII215.

Published

Journal Article

Several P element insertion and deletion mutations near the 5' end of Drosophila melanogaster RpII215 have been examined by nucleotide sequencing. Two different sites of P element insertion, approximately 90 nucleotides apart, have been detected in this region of the gene. Therefore, including an additional site of P element insertion within the coding region, there are at least three distinct sites of P element insertion at RpII215. Both 5' sites are within a noncoding portion of transcribed sequences. The sequences of four revertants of one P element insertion mutation (D50) indicate that the P element is either precisely deleted or internally deleted to restore RpII215 activity. Partial internal deletions of the P element result in different RpII215 activity levels, which appear to depend on the specific sequences that remain after excision.

Full Text

Duke Authors

Cited Authors

  • Searles, LL; Greenleaf, AL; Kemp, WE; Voelker, RA

Published Date

  • October 1, 1986

Published In

Volume / Issue

  • 6 / 10

Start / End Page

  • 3312 - 3319

PubMed ID

  • 3025586

Pubmed Central ID

  • 3025586

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.6.10.3312

Language

  • eng

Conference Location

  • United States