An activity necessary for in vitro transcription is a DNase inhibitor.

Published

Journal Article

A phosphocellulose flowthrough fraction required for accurate transcription in vitro by RNA polymerase II was found to contain a DNase inhibitor which was necessary to maintain template integrity (Price D.H., Sluder A.E. & Greenleaf A.L. (1987) J. Biol. Chem. 262, 3244-3255). Starting with a Drosophila Kc cell nuclear extract, the DNase inhibitory activity has been purified 19,000-fold. In combination with the other necessary fractions, the highly purified inhibitor continues to support reconstruction of transcription. It thus appears to be the only required activity in the original phosphocellulose flowthrough fraction. The inhibitor is a protein which does not bind to DNA or inhibit DNase I, so that it has also been useful in assays for DNA binding proteins in crude, DNase-contaminated fractions.

Full Text

Duke Authors

Cited Authors

  • Sluder, AE; Price, DH; Greenleaf, AL

Published Date

  • November 1987

Published In

Volume / Issue

  • 69 / 11-12

Start / End Page

  • 1199 - 1205

PubMed ID

  • 3129025

Pubmed Central ID

  • 3129025

International Standard Serial Number (ISSN)

  • 0300-9084

Digital Object Identifier (DOI)

  • 10.1016/0300-9084(87)90147-7

Language

  • eng

Conference Location

  • France