Assaying CTD kinases in vitro and phosphorylation-modulated properties of RNA polymerase II in vivo.
Journal Article (Review)
The functional properties of RNA polymerase II are modulated by hyperphosphorylation of its unique C-terminal repeat domain (CTD). A number of enzymes with CTD kinase activity have been identified, and correlations between CTD phosphorylation and RNA polymerase II function have been made. Here we describe methods for assaying CTD kinases and for characterizing them enzymologically. In addition we present approaches for studying phosphorylation-mediated behavior of chromosome-associated RNA polymerase II by using CTD-directed, phosphorylation state-sensitive antibodies and in situ localization techniques. The methods described here should, in conjunction with genetic approaches, contribute to elucidating the physiological roles of CTD kinases.
Full Text
Duke Authors
Cited Authors
- Morris, DP; Lee, JM; Sterner, DE; Brickey, WJ; Greenleaf, AL
Published Date
- July 1997
Published In
Volume / Issue
- 12 / 3
Start / End Page
- 264 - 275
PubMed ID
- 9237170
Pubmed Central ID
- 9237170
Electronic International Standard Serial Number (EISSN)
- 1095-9130
International Standard Serial Number (ISSN)
- 1046-2023
Digital Object Identifier (DOI)
- 10.1006/meth.1997.0478
Language
- eng