Assaying CTD kinases in vitro and phosphorylation-modulated properties of RNA polymerase II in vivo.

Published

Journal Article (Review)

The functional properties of RNA polymerase II are modulated by hyperphosphorylation of its unique C-terminal repeat domain (CTD). A number of enzymes with CTD kinase activity have been identified, and correlations between CTD phosphorylation and RNA polymerase II function have been made. Here we describe methods for assaying CTD kinases and for characterizing them enzymologically. In addition we present approaches for studying phosphorylation-mediated behavior of chromosome-associated RNA polymerase II by using CTD-directed, phosphorylation state-sensitive antibodies and in situ localization techniques. The methods described here should, in conjunction with genetic approaches, contribute to elucidating the physiological roles of CTD kinases.

Full Text

Duke Authors

Cited Authors

  • Morris, DP; Lee, JM; Sterner, DE; Brickey, WJ; Greenleaf, AL

Published Date

  • July 1997

Published In

Volume / Issue

  • 12 / 3

Start / End Page

  • 264 - 275

PubMed ID

  • 9237170

Pubmed Central ID

  • 9237170

Electronic International Standard Serial Number (EISSN)

  • 1095-9130

International Standard Serial Number (ISSN)

  • 1046-2023

Digital Object Identifier (DOI)

  • 10.1006/meth.1997.0478

Language

  • eng