RNA polymerase B (or II) in heat induced puffs of Drosophila polytene chromosomes.

Journal Article

Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff displayed intense fluorescence when stained for RNA polymerase B, but relatively little fluorescence when stained for histone H1. Returning heat shocked larvae to room temperature resulted in the appearance of a distinctive pattern of RNA polymerase-associated fluorescence in the heat shock puff at 87C, presumably reflecting events associated with the inactivation and regression of this puff. Large differences observed in the apparent RNA polymerase B content of puffs of similar size suggest that the interaction of RNA polymerase B with chromosomal loci does not depend on simply the state of condensation or decondensation of the chromatin.

Full Text

Duke Authors

Cited Authors

  • Greenleaf, AL; Plagens, U; Jamrich, M; Bautz, EK

Published Date

  • January 16, 1978

Published In

Volume / Issue

  • 65 / 2

Start / End Page

  • 127 - 136

PubMed ID

  • 414900

International Standard Serial Number (ISSN)

  • 0009-5915

Language

  • eng

Conference Location

  • Austria