Replication timing of DNA sequences associated with human centromeres and telomeres.


Journal Article

The timing of replication of centromere-associated human alpha satellite DNA from chromosomes X, 17, and 7 as well as of human telomeric sequences was determined by using density-labeling methods and fluorescence-activated cell sorting. Alpha satellite sequences replicated late in S phase; however, the alpha satellite sequences of the three chromosomes studied replicated at slightly different times. Human telomeres were found to replicate throughout most of S phase. These results are consistent with a model in which multiple initiations of replication occur at a characteristic time within the alpha satellite repeats of a particular chromosome, while the replication timing of telomeric sequences is determined by either telomeric origins that can initiate at different times during S phase or by replication origins within the flanking chromosomal DNA sequences.

Full Text

Cited Authors

  • Ten Hagen, KG; Gilbert, DM; Willard, HF; Cohen, SN

Published Date

  • December 1990

Published In

Volume / Issue

  • 10 / 12

Start / End Page

  • 6348 - 6355

PubMed ID

  • 2247059

Pubmed Central ID

  • 2247059

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.10.12.6348


  • eng