Pulsed-field gel analysis of alpha-satellite DNA at the human X chromosome centromere: high-frequency polymorphisms and array size estimate.

Published

Journal Article

Using pulsed-field gel analysis (PFGE), we have characterized the large array of alpha-satellite DNA located in the centromeric region of the human X chromosome. The tandem repetitive nature of this DNA family lends itself to examination by PFGE using restriction enzymes that cleave frequently in unique sequence DNA but which cut only rarely within the repetitive alpha-satellite array. Several such restriction enzymes (BglI, BglII, KpnI, ScaI) have proven highly informative in sizing the alpha-satellite array and in following the segregation of individual X-chromosome centromeres using PFGE polymorphisms. Among 29 different X chromosomes, alpha-satellite array length varied between 1380 and 3730 kb (mean = 2895 kb; SD = 537). In three large CEPH families comprising 24 meioses, inheritance of these PFGE polymorphisms was strictly Mendelian, with no indication of intraarray recombination. Such DXZ1 alpha-satellite polymorphisms, therefore, may prove useful in the study of pericentromeric X-linked disorders.

Full Text

Cited Authors

  • Mahtani, MM; Willard, HF

Published Date

  • August 1990

Published In

Volume / Issue

  • 7 / 4

Start / End Page

  • 607 - 613

PubMed ID

  • 1974881

Pubmed Central ID

  • 1974881

Electronic International Standard Serial Number (EISSN)

  • 1089-8646

International Standard Serial Number (ISSN)

  • 0888-7543

Digital Object Identifier (DOI)

  • 10.1016/0888-7543(90)90206-a

Language

  • eng