A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status.


Journal Article

A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3-kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3-kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE1 escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.

Full Text

Cited Authors

  • Coleman, MP; Ambrose, HJ; Carrel, L; Németh, AH; Willard, HF; Davies, KE

Published Date

  • January 1, 1996

Published In

Volume / Issue

  • 31 / 1

Start / End Page

  • 135 - 138

PubMed ID

  • 8808293

Pubmed Central ID

  • 8808293

Electronic International Standard Serial Number (EISSN)

  • 1089-8646

International Standard Serial Number (ISSN)

  • 0888-7543

Digital Object Identifier (DOI)

  • 10.1006/geno.1996.0022


  • eng