Gene on short arm of human X chromosome complements murine tsA1S9 DNA synthesis mutation.


Journal Article

We have created somatic cell hybrids between the temperature-sensitive mouse cell line tsA1S9 and human cell lines in order to localize the human gene (A1S9T) complementing the cell cycle defect of the murine line. Segregation of the human X chromosome is completely concordant with growth at the nonpermissive temperature. Hybrids retaining the X chromosome are temperature-resistant, whereas those without a human X are temperature-sensitive. Further hybrids made using human cell lines with X-autosome translocations indicate that the A1S9T gene is located on the short arm of the human X chromosome.

Full Text

Cited Authors

  • Brown, CJ; Powers, VE; Munroe, DL; Sheinin, R; Willard, HF

Published Date

  • March 1989

Published In

Volume / Issue

  • 15 / 2

Start / End Page

  • 173 - 178

PubMed ID

  • 2928842

Pubmed Central ID

  • 2928842

International Standard Serial Number (ISSN)

  • 0740-7750

Digital Object Identifier (DOI)

  • 10.1007/bf01535079


  • eng