BrdU-33258 Hoechst analysis of DNA replication in human lymphocytes with supernumerary or structurally abnormal X chromosomes.


Journal Article

BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolution method for determining the interchange points of X-X and X-autosome translocations. Asynchrony among terminal replication patterns of multiple late replicating X chromosomes within an individual cell can occasionally be demonstrated. The arms of isochromosomes usually exhibit symmetrical fluorescence patterns, with replication terminating in bands Xq21 and Xq23 (predominant pattern) or in bands Xq25 and Xq27 (alternative pattern) in both arms. In the vast majority of lymphocytes containing a balanced X-13 or X-19 translocation, the normal X is late replicating. However, DNA synthesis in the translocation products occasionally appears somewhat delayed relative to that expected for an early replicating X, consistent with possible position effects on replication kinetics.

Full Text

Cited Authors

  • Latt, SA; Willard, HF; Gerald, PS

Published Date

  • August 1, 1976

Published In

Volume / Issue

  • 57 / 2

Start / End Page

  • 135 - 153

PubMed ID

  • 954550

Pubmed Central ID

  • 954550

Electronic International Standard Serial Number (EISSN)

  • 1432-0886

International Standard Serial Number (ISSN)

  • 0009-5915

Digital Object Identifier (DOI)

  • 10.1007/bf00292912


  • eng