Molecular cloning and chromosomal localization of DNA sequences associated with a human DNA repair gene.


Journal Article

The genes and gene products involved in the mammalian DNA repair processes have yet to be identified. Toward this end we made use of a number of DNA repair-proficient transformants that were generated after transfection of DNA from repair-proficient human cells into a mutant hamster line that is defective in the initial incision step of the excision repair process. In this report, biochemical evidence is presented that demonstrates that these transformants are repair proficient. In addition, we describe the molecular identification and cloning of unique DNA sequences closely associated with the transfected human DNA repair gene and demonstrate the presence of homologous DNA sequences in human cells and in the repair-proficient DNA transformants. The chromosomal location of these sequences was determined by using a panel of rodent-human somatic cell hybrids. Both unique DNA sequences were found to be on human chromosome 19.

Full Text

Cited Authors

  • Rubin, JS; Prideaux, VR; Willard, HF; Dulhanty, AM; Whitmore, GF; Bernstein, A

Published Date

  • February 1985

Published In

Volume / Issue

  • 5 / 2

Start / End Page

  • 398 - 405

PubMed ID

  • 2983193

Pubmed Central ID

  • 2983193

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.5.2.398


  • eng