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Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2.

Publication ,  Journal Article
Greenberg, CS; Hursting, MJ; Macik, BG; Ortel, TL; Kane, WH; Moore, BM
Published in: Clin Chem
October 1994

We have used a monoclonal antibody-based ELISA for plasma prothrombin fragment 1.2 (F1.2) to establish appropriate sample collection and storage conditions for this biomarker of thrombin generation. F1.2 concentrations were not altered by exogenous factor Xa, thrombin, or thromboplastin if blood was collected by routine venipuncture into tubes containing heparin as anticoagulant (but not citrate, acid-citrate-dextrose, EDTA, or oxalate) and if plasma antithrombin III concentration was > or = 30% of normal. Heparinized plasma F1.2 was stable for > or = 8 h at 20-25 degrees C, and if premixed with a stabilizing reagent, for > or = 4 years at -70 degrees C. Mean values for heparinized plasma F1.2 collected and stored by recommended procedures were increased in patients with thrombosis and conditions of increased thrombotic risk, and were sensitive to heparin and oral anticoagulant therapies. We conclude that plasma obtained by routine venipuncture into tubes with heparin as anticoagulant is an appropriate specimen for F1.2 measurements for most patients.

Duke Scholars

Published In

Clin Chem

ISSN

0009-9147

Publication Date

October 1994

Volume

40

Issue

10

Start / End Page

1962 / 1969

Location

England

Related Subject Headings

  • Thrombosis
  • Reference Values
  • Prothrombin
  • Platelet Count
  • Platelet Aggregation
  • Peptide Fragments
  • Middle Aged
  • Humans
  • Heparin
  • General Clinical Medicine
 

Citation

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Greenberg, C. S., Hursting, M. J., Macik, B. G., Ortel, T. L., Kane, W. H., & Moore, B. M. (1994). Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2. Clin Chem, 40(10), 1962–1969.
Greenberg, C. S., M. J. Hursting, B. G. Macik, T. L. Ortel, W. H. Kane, and B. M. Moore. “Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2.Clin Chem 40, no. 10 (October 1994): 1962–69.
Greenberg CS, Hursting MJ, Macik BG, Ortel TL, Kane WH, Moore BM. Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2. Clin Chem. 1994 Oct;40(10):1962–9.
Greenberg, C. S., et al. “Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2.Clin Chem, vol. 40, no. 10, Oct. 1994, pp. 1962–69.
Greenberg CS, Hursting MJ, Macik BG, Ortel TL, Kane WH, Moore BM. Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2. Clin Chem. 1994 Oct;40(10):1962–1969.

Published In

Clin Chem

ISSN

0009-9147

Publication Date

October 1994

Volume

40

Issue

10

Start / End Page

1962 / 1969

Location

England

Related Subject Headings

  • Thrombosis
  • Reference Values
  • Prothrombin
  • Platelet Count
  • Platelet Aggregation
  • Peptide Fragments
  • Middle Aged
  • Humans
  • Heparin
  • General Clinical Medicine