Silver staining of histones in Triton-acid-urea gels.
Publication
, Journal Article
Mold, DE; Weingart, J; Assaraf, J; Lubahn, DB; Kelner, DN; Shaw, BR; McCarty, KS
Published in: Analytical biochemistry
November 1983
A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.
Duke Scholars
Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
November 1983
Volume
135
Issue
1
Start / End Page
44 / 47
Related Subject Headings
- Urea
- Thymus Gland
- Staining and Labeling
- Silver
- Polyethylene Glycols
- Octoxynol
- Histones
- Gels
- Electrophoresis
- Cattle
Citation
APA
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ICMJE
MLA
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Mold, D. E., Weingart, J., Assaraf, J., Lubahn, D. B., Kelner, D. N., Shaw, B. R., & McCarty, K. S. (1983). Silver staining of histones in Triton-acid-urea gels. Analytical Biochemistry, 135(1), 44–47. https://doi.org/10.1016/0003-2697(83)90727-3
Mold, D. E., J. Weingart, J. Assaraf, D. B. Lubahn, D. N. Kelner, B. R. Shaw, and K. S. McCarty. “Silver staining of histones in Triton-acid-urea gels.” Analytical Biochemistry 135, no. 1 (November 1983): 44–47. https://doi.org/10.1016/0003-2697(83)90727-3.
Mold DE, Weingart J, Assaraf J, Lubahn DB, Kelner DN, Shaw BR, et al. Silver staining of histones in Triton-acid-urea gels. Analytical biochemistry. 1983 Nov;135(1):44–7.
Mold, D. E., et al. “Silver staining of histones in Triton-acid-urea gels.” Analytical Biochemistry, vol. 135, no. 1, Nov. 1983, pp. 44–47. Epmc, doi:10.1016/0003-2697(83)90727-3.
Mold DE, Weingart J, Assaraf J, Lubahn DB, Kelner DN, Shaw BR, McCarty KS. Silver staining of histones in Triton-acid-urea gels. Analytical biochemistry. 1983 Nov;135(1):44–47.
Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
November 1983
Volume
135
Issue
1
Start / End Page
44 / 47
Related Subject Headings
- Urea
- Thymus Gland
- Staining and Labeling
- Silver
- Polyethylene Glycols
- Octoxynol
- Histones
- Gels
- Electrophoresis
- Cattle