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Regulated expression of p18, a major phosphoprotein of leukemic cells.

Publication ,  Journal Article
Luo, XN; Arcasoy, MO; Brickner, HE; Mistry, S; Schechter, AD; Atweh, GF
Published in: J Biol Chem
November 5, 1991

p18 is a phosphoprotein that is present in great abundance in acute leukemia blasts and in less abundance in proliferating lymphocytes. This protein undergoes major changes in its state of phosphorylation upon induction of differentiation of leukemic cells in culture. The same protein appears to be involved in a variety of other cellular processes that include regulation of hormone secretion, T cell activation, muscle differentiation, and brain development. In this report, we describe our studies of the regulation of expression of this gene in leukemic cells. We show that the expression of this gene is markedly reduced upon induction of differentiation of a variety of leukemic cells in culture. We use a cDNA clone that we constructed earlier which encodes this protein as a probe to isolate the human chromosomal p18 gene. We characterize the 5' end of this gene in detail and identify its promoter element. We also identify a regulatory element in the first intervening sequence (IVS-1) of this gene which loses its DNase I hypersensitivity upon induction of differentiation of leukemic cells in culture. Our DNase I footprinting experiments demonstrate nuclear protein binding to multiple sequence motifs within its promoter element and its IVS-1 regulatory element. Functional studies using a transient expression system show that deletion of these sequence motifs has profound effects on the expression of this gene. These studies begin to shed some light on the mechanism of regulation of a gene that may be involved in control of cell growth and differentiation and in a variety of other vital cellular processes.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 5, 1991

Volume

266

Issue

31

Start / End Page

21004 / 21010

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Regulatory Sequences, Nucleic Acid
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Phosphoproteins
  • Molecular Sequence Data
  • Leukemia
  • Introns
  • In Vitro Techniques
  • Humans
 

Citation

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Luo, X. N., Arcasoy, M. O., Brickner, H. E., Mistry, S., Schechter, A. D., & Atweh, G. F. (1991). Regulated expression of p18, a major phosphoprotein of leukemic cells. J Biol Chem, 266(31), 21004–21010.
Luo, X. N., M. O. Arcasoy, H. E. Brickner, S. Mistry, A. D. Schechter, and G. F. Atweh. “Regulated expression of p18, a major phosphoprotein of leukemic cells.J Biol Chem 266, no. 31 (November 5, 1991): 21004–10.
Luo XN, Arcasoy MO, Brickner HE, Mistry S, Schechter AD, Atweh GF. Regulated expression of p18, a major phosphoprotein of leukemic cells. J Biol Chem. 1991 Nov 5;266(31):21004–10.
Luo, X. N., et al. “Regulated expression of p18, a major phosphoprotein of leukemic cells.J Biol Chem, vol. 266, no. 31, Nov. 1991, pp. 21004–10.
Luo XN, Arcasoy MO, Brickner HE, Mistry S, Schechter AD, Atweh GF. Regulated expression of p18, a major phosphoprotein of leukemic cells. J Biol Chem. 1991 Nov 5;266(31):21004–21010.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 5, 1991

Volume

266

Issue

31

Start / End Page

21004 / 21010

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Regulatory Sequences, Nucleic Acid
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Phosphoproteins
  • Molecular Sequence Data
  • Leukemia
  • Introns
  • In Vitro Techniques
  • Humans