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Expression of erythropoietin receptor splice variants in human cancer.

Publication ,  Journal Article
Arcasoy, MO; Jiang, X; Haroon, ZA
Published in: Biochem Biophys Res Commun
August 8, 2003

Erythropoietin (EPO) regulates mammalian erythropoiesis by binding to its transmembrane receptor EPOR. Recent studies demonstrated functional EPOR expression in human cancer cells. Recombinant human EPO was reported to stimulate the proliferation of monolayer cultures of breast and renal carcinoma cells. Furthermore, administration of EPO-EPOR antagonists delayed the growth of uterine, ovarian, and mammary carcinoma cells in experimental animal models. In this study, we show EPOR transcript and protein expression in breast, colon, lung, ovary, and prostate cancer cells. Using reverse transcription-polymerase chain reaction, we isolated and characterized several novel cDNAs for EPOR splice variants expressed in cancer cells. Deduced amino acid sequences of the cDNAs revealed splice variants encoding soluble EPOR or membrane-bound EPOR peptides with intra-cytoplasmic, carboxy-terminal truncations. These findings indicate the expression of multiple EPOR isoforms in human cancer cells that may modulate the cellular effects of recombinant human EPO or EPO-EPOR antagonists.

Duke Scholars

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 8, 2003

Volume

307

Issue

4

Start / End Page

999 / 1007

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Receptors, Erythropoietin
  • RNA, Messenger
  • Protein Isoforms
  • Neoplasms
  • Molecular Sequence Data
  • Humans
  • Cricetinae
  • CHO Cells
 

Citation

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Arcasoy, M. O., Jiang, X., & Haroon, Z. A. (2003). Expression of erythropoietin receptor splice variants in human cancer. Biochem Biophys Res Commun, 307(4), 999–1007. https://doi.org/10.1016/s0006-291x(03)01303-2
Arcasoy, Murat O., Xiaohong Jiang, and Zishan A. Haroon. “Expression of erythropoietin receptor splice variants in human cancer.Biochem Biophys Res Commun 307, no. 4 (August 8, 2003): 999–1007. https://doi.org/10.1016/s0006-291x(03)01303-2.
Arcasoy MO, Jiang X, Haroon ZA. Expression of erythropoietin receptor splice variants in human cancer. Biochem Biophys Res Commun. 2003 Aug 8;307(4):999–1007.
Arcasoy, Murat O., et al. “Expression of erythropoietin receptor splice variants in human cancer.Biochem Biophys Res Commun, vol. 307, no. 4, Aug. 2003, pp. 999–1007. Pubmed, doi:10.1016/s0006-291x(03)01303-2.
Arcasoy MO, Jiang X, Haroon ZA. Expression of erythropoietin receptor splice variants in human cancer. Biochem Biophys Res Commun. 2003 Aug 8;307(4):999–1007.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 8, 2003

Volume

307

Issue

4

Start / End Page

999 / 1007

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Receptors, Erythropoietin
  • RNA, Messenger
  • Protein Isoforms
  • Neoplasms
  • Molecular Sequence Data
  • Humans
  • Cricetinae
  • CHO Cells