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Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein.

Publication ,  Journal Article
Watkins, PJ; Condreay, JP; Huber, BE; Jacobs, SJ; Adams, DJ
Published in: Cancer Res
March 1, 1996

A plasmid containing the CCAAT/enhancer-binding protein (C/EBP alpha) gene transcriptionally controlled by the metallothionein promoter was constructed. The gene was transfected into the human hepatocellular carcinoma cell lines Hep3B and HepG2. When cultured in vitro in the presence of 100 microM ZnSO(4), C/EBP alpha expression caused reversible growth arrest. In soft agar clonogenic assays, C/EBP alpha expression decreased both the colony size and the total number of colonies compared with zinc-free controls. C/EBP alpha expressing cells s.c. implanted in CD-1 nu/nu mice were essentially nontumorigenic, whereas C/EBP alpha tumor cells implanted into immunodeficient SCID mice demonstrated a significantly delayed time of tumor appearance compared with cells transfected with a vector control plasmid. These studies suggest that the expression of endogenous genes normally associated with a quiescent, differentiated state, such as C/EBP alpha, can result in impaired proliferative activity and suppressed tumorigenicity of hepatoma cell lines.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 1, 1996

Volume

56

Issue

5

Start / End Page

1063 / 1067

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Liver Neoplasms
  • Humans
  • Gene Transfer Techniques
  • Gene Expression Regulation, Neoplastic
  • DNA-Binding Proteins
 

Citation

APA
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ICMJE
MLA
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Watkins, P. J., Condreay, J. P., Huber, B. E., Jacobs, S. J., & Adams, D. J. (1996). Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. Cancer Res, 56(5), 1063–1067.
Watkins, P. J., J. P. Condreay, B. E. Huber, S. J. Jacobs, and D. J. Adams. “Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein.Cancer Res 56, no. 5 (March 1, 1996): 1063–67.
Watkins PJ, Condreay JP, Huber BE, Jacobs SJ, Adams DJ. Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. Cancer Res. 1996 Mar 1;56(5):1063–7.
Watkins, P. J., et al. “Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein.Cancer Res, vol. 56, no. 5, Mar. 1996, pp. 1063–67.
Watkins PJ, Condreay JP, Huber BE, Jacobs SJ, Adams DJ. Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. Cancer Res. 1996 Mar 1;56(5):1063–1067.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 1, 1996

Volume

56

Issue

5

Start / End Page

1063 / 1067

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Liver Neoplasms
  • Humans
  • Gene Transfer Techniques
  • Gene Expression Regulation, Neoplastic
  • DNA-Binding Proteins