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A new in vivo method for quantitative analysis of stroke lesions using diffusion-weighted magnetic resonance microscopy.

Publication ,  Journal Article
Hall, WL; Benveniste, H; Hedlund, LW; Johnson, GA
Published in: Neuroimage
June 1996

Using three-dimensional diffusion-weighted MR microscopy and a rat model of focal cerebral ischemia, we evaluated the statistical characteristics of two parameters: absolute stroke volumes and change in stroke volumes over 6 h of middle cerebral artery (MCA) occlusion. In all rats, the absolute stroke volumes increased linearly over the 6-h MCA occlusion time period. On average, stroke volume growth rate was 2.1 +/- 0.5%/h. Sample size power analysis of our data demonstrated that to demonstrate a 10% reduction of the 6-h volumes, sample size per group would require 29 animals (these calculations are based on alpha = 0.05, beta = 0.20 using normal approximation). A similar 30% reduction of stroke volume at 6 h poststroke (from approximately equal to 200 to 140 mm3) would, in our "slope model," translate into a reduction of stroke growth rate from the normal + 11.25 mm3/h (150 to 200 mm3 over 4 h) to 7 mm3/h (150 to 178 mm3 over 4 h); power analysis in this case demonstrated that sample size is reduced to 15 animals per group (these calculations are based on alpha = 0.05, beta = 0.20 using normal approximation). We conclude that from a statistical standpoint our study demonstrates that stroke growth rate might be a more suitable parameter for evaluating the effect of treatment in both clinical and experimental stroke trials.

Duke Scholars

Published In

Neuroimage

DOI

ISSN

1053-8119

Publication Date

June 1996

Volume

3

Issue

3 Pt 1

Start / End Page

158 / 166

Location

United States

Related Subject Headings

  • Stroke Volume
  • Rats, Inbred F344
  • Rats
  • Neurology & Neurosurgery
  • Models, Neurological
  • Microscopy
  • Magnetic Resonance Imaging
  • Female
  • Disease Models, Animal
  • Brain Ischemia
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hall, W. L., Benveniste, H., Hedlund, L. W., & Johnson, G. A. (1996). A new in vivo method for quantitative analysis of stroke lesions using diffusion-weighted magnetic resonance microscopy. Neuroimage, 3(3 Pt 1), 158–166. https://doi.org/10.1006/nimg.1996.0017
Hall, W. L., H. Benveniste, L. W. Hedlund, and G. A. Johnson. “A new in vivo method for quantitative analysis of stroke lesions using diffusion-weighted magnetic resonance microscopy.Neuroimage 3, no. 3 Pt 1 (June 1996): 158–66. https://doi.org/10.1006/nimg.1996.0017.
Hall WL, Benveniste H, Hedlund LW, Johnson GA. A new in vivo method for quantitative analysis of stroke lesions using diffusion-weighted magnetic resonance microscopy. Neuroimage. 1996 Jun;3(3 Pt 1):158–66.
Hall, W. L., et al. “A new in vivo method for quantitative analysis of stroke lesions using diffusion-weighted magnetic resonance microscopy.Neuroimage, vol. 3, no. 3 Pt 1, June 1996, pp. 158–66. Pubmed, doi:10.1006/nimg.1996.0017.
Hall WL, Benveniste H, Hedlund LW, Johnson GA. A new in vivo method for quantitative analysis of stroke lesions using diffusion-weighted magnetic resonance microscopy. Neuroimage. 1996 Jun;3(3 Pt 1):158–166.
Journal cover image

Published In

Neuroimage

DOI

ISSN

1053-8119

Publication Date

June 1996

Volume

3

Issue

3 Pt 1

Start / End Page

158 / 166

Location

United States

Related Subject Headings

  • Stroke Volume
  • Rats, Inbred F344
  • Rats
  • Neurology & Neurosurgery
  • Models, Neurological
  • Microscopy
  • Magnetic Resonance Imaging
  • Female
  • Disease Models, Animal
  • Brain Ischemia