A defined human system that supports bidirectional mismatch-provoked excision.

Journal Article (Journal Article)

Mismatch-provoked excision directed by a strand break located 3' or 5' to the mispair has been reconstituted using purified human proteins. While MutSalpha, EXOI, and RPA are sufficient to support hydrolysis directed by a 5' strand break, 3' directed excision also requires MutLalpha, PCNA, and RFC. EXOI interacts with PCNA. RFC and PCNA suppress EXOI-mediated 5' to 3' hydrolysis when the nick that directs excision is located 3' to the mispair and activate 3' to 5' excision, which is dependent on loaded PCNA and apparently mediated by a cryptic EXOI 3' to 5' hydrolytic function. By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break.

Full Text

Duke Authors

Cited Authors

  • Dzantiev, L; Constantin, N; Genschel, J; Iyer, RR; Burgers, PM; Modrich, P

Published Date

  • July 2, 2004

Published In

Volume / Issue

  • 15 / 1

Start / End Page

  • 31 - 41

PubMed ID

  • 15225546

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2004.06.016


  • eng

Conference Location

  • United States