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A defined human system that supports bidirectional mismatch-provoked excision.

Publication ,  Journal Article
Dzantiev, L; Constantin, N; Genschel, J; Iyer, RR; Burgers, PM; Modrich, P
Published in: Mol Cell
July 2, 2004

Mismatch-provoked excision directed by a strand break located 3' or 5' to the mispair has been reconstituted using purified human proteins. While MutSalpha, EXOI, and RPA are sufficient to support hydrolysis directed by a 5' strand break, 3' directed excision also requires MutLalpha, PCNA, and RFC. EXOI interacts with PCNA. RFC and PCNA suppress EXOI-mediated 5' to 3' hydrolysis when the nick that directs excision is located 3' to the mispair and activate 3' to 5' excision, which is dependent on loaded PCNA and apparently mediated by a cryptic EXOI 3' to 5' hydrolytic function. By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break.

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Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

July 2, 2004

Volume

15

Issue

1

Start / End Page

31 / 41

Location

United States

Related Subject Headings

  • Replication Protein A
  • Proteins
  • Proliferating Cell Nuclear Antigen
  • MutL Proteins
  • Hydrolysis
  • Humans
  • Hela Cells
  • HeLa Cells
  • Exodeoxyribonucleases
  • Developmental Biology
 

Citation

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Dzantiev, L., Constantin, N., Genschel, J., Iyer, R. R., Burgers, P. M., & Modrich, P. (2004). A defined human system that supports bidirectional mismatch-provoked excision. Mol Cell, 15(1), 31–41. https://doi.org/10.1016/j.molcel.2004.06.016
Dzantiev, Leonid, Nicoleta Constantin, Jochen Genschel, Ravi R. Iyer, Peter M. Burgers, and Paul Modrich. “A defined human system that supports bidirectional mismatch-provoked excision.Mol Cell 15, no. 1 (July 2, 2004): 31–41. https://doi.org/10.1016/j.molcel.2004.06.016.
Dzantiev L, Constantin N, Genschel J, Iyer RR, Burgers PM, Modrich P. A defined human system that supports bidirectional mismatch-provoked excision. Mol Cell. 2004 Jul 2;15(1):31–41.
Dzantiev, Leonid, et al. “A defined human system that supports bidirectional mismatch-provoked excision.Mol Cell, vol. 15, no. 1, July 2004, pp. 31–41. Pubmed, doi:10.1016/j.molcel.2004.06.016.
Dzantiev L, Constantin N, Genschel J, Iyer RR, Burgers PM, Modrich P. A defined human system that supports bidirectional mismatch-provoked excision. Mol Cell. 2004 Jul 2;15(1):31–41.
Journal cover image

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

July 2, 2004

Volume

15

Issue

1

Start / End Page

31 / 41

Location

United States

Related Subject Headings

  • Replication Protein A
  • Proteins
  • Proliferating Cell Nuclear Antigen
  • MutL Proteins
  • Hydrolysis
  • Humans
  • Hela Cells
  • HeLa Cells
  • Exodeoxyribonucleases
  • Developmental Biology