The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor

Journal Article

In most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the A and B mating-type loci). In this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom Pleurotus djamor using both positional cloning and degenerate PCR methods. DNA sequences from genomic regions cosegregating with the mating-type loci of P. djamor revealed homeodomain transcription factors (A) and pheromone receptors (B), suggesting the genetic basis for mating-type determination in P. djamor is the same as in the model mushroom species. Three pheromone receptors were detected in a single homokaryotic isolate of P. djamor. Only one pair of homeodomain genes was detected in the A mating-type region. It is hypothesized that the A mating-type locus of P. djamor is comprised of only one homeodomain pair, which may explain the lower number of A mating-type alleles relative to other mushroom species. © 2004 Elsevier Inc. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • James, TY; Liou, S-R; Vilgalys, R

Published Date

  • 2004

Published In

  • Fungal Genetics and Biology

Volume / Issue

  • 41 / 8

Start / End Page

  • 813 - 825

PubMed ID

  • 15219565

Digital Object Identifier (DOI)

  • 10.1016/j.fgb.2004.04.005