Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi

Journal Article

The high level of DNA polymorphism at the mating-type loci of mushroom fungi has made the cloning of mating-type genes difficult. As an alternative to strategies that employ sequence conservation, an approach utilizing conserved gene order could facilitate the cloning of A mating-type genes from mushroom fungi. It has been shown that a gene encoding a mitochondrial intermediate peptidase (MIP) is very close (<1kbp) to the A mating-type locus of two model mushroom species. In this study, the cosegregation of MIP and the A mating-type locus was studied by genotyping progeny of seven additional mushroom species using PCR and genetic crosses. No evidence of any recombination between MIP and the A mating-type locus was detected among all seven species. Phylogenetic analysis of MIP sequences from diverse mushroom species agrees with the current organismal phylogeny, suggesting the sequences are generally orthologous. © 2003 Elsevier Inc. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • James, TY; Kües, U; Rehner, SA; Vilgalys, R

Published Date

  • 2004

Published In

  • Fungal Genetics and Biology

Volume / Issue

  • 41 / 3

Start / End Page

  • 381 - 390

PubMed ID

  • 14761798

Digital Object Identifier (DOI)

  • 10.1016/j.fgb.2003.11.008