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Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta.

Publication ,  Journal Article
Wong, C; Rougier-Chapman, EM; Frederick, JP; Datto, MB; Liberati, NT; Li, JM; Wang, XF
Published in: Mol Cell Biol
March 1999

Transcriptional regulation by transforming growth factor beta (TGF-beta) is a complex process which is likely to involve cross talk between different DNA responsive elements and transcription factors to achieve maximal promoter activation and specificity. Here, we describe a concurrent requirement for two discrete responsive elements in the regulation of the c-Jun promoter, one a binding site for a Smad3-Smad4 complex and the other an AP-1 binding site. The two elements are located 120 bp apart in the proximal c-Jun promoter, and each was able to independently bind its corresponding transcription factor complex. The effects of independently mutating each of these elements were nonadditive; disruption of either sequence resulted in complete or severe reductions in TGF-beta responsiveness. This simultaneous requirement for two distinct and independent DNA binding elements suggests that Smad and AP-1 complexes function synergistically to mediate TGF-beta-induced transcriptional activation of the c-Jun promoter.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

March 1999

Volume

19

Issue

3

Start / End Page

1821 / 1830

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Transcriptional Activation
  • Transcription Factor AP-1
  • Trans-Activators
  • TATA Box
  • Smad4 Protein
  • Smad3 Protein
  • Rabbits
  • Proto-Oncogene Proteins c-jun
  • Promoter Regions, Genetic
 

Citation

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Chicago
ICMJE
MLA
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Wong, C., Rougier-Chapman, E. M., Frederick, J. P., Datto, M. B., Liberati, N. T., Li, J. M., & Wang, X. F. (1999). Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta. Mol Cell Biol, 19(3), 1821–1830. https://doi.org/10.1128/MCB.19.3.1821
Wong, C., E. M. Rougier-Chapman, J. P. Frederick, M. B. Datto, N. T. Liberati, J. M. Li, and X. F. Wang. “Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta.Mol Cell Biol 19, no. 3 (March 1999): 1821–30. https://doi.org/10.1128/MCB.19.3.1821.
Wong C, Rougier-Chapman EM, Frederick JP, Datto MB, Liberati NT, Li JM, et al. Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta. Mol Cell Biol. 1999 Mar;19(3):1821–30.
Wong, C., et al. “Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta.Mol Cell Biol, vol. 19, no. 3, Mar. 1999, pp. 1821–30. Pubmed, doi:10.1128/MCB.19.3.1821.
Wong C, Rougier-Chapman EM, Frederick JP, Datto MB, Liberati NT, Li JM, Wang XF. Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta. Mol Cell Biol. 1999 Mar;19(3):1821–1830.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

March 1999

Volume

19

Issue

3

Start / End Page

1821 / 1830

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Transcriptional Activation
  • Transcription Factor AP-1
  • Trans-Activators
  • TATA Box
  • Smad4 Protein
  • Smad3 Protein
  • Rabbits
  • Proto-Oncogene Proteins c-jun
  • Promoter Regions, Genetic