Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity.

Published

Journal Article

Because hematopoietic stem cells are rich in aldehyde dehydrogenase (ALDH) activity, we developed a fluorescent substrate for ALDH, termed BODIPY aminoacetaldehyde (BAAA), and tested its potential for isolating primitive human hematopoietic cells. A population of cells with low orthogonal light scattering and bright fluorescence intensity (SSC(lo)ALDH(br) cells) could be readily fractionated from human umbilical cord blood cells costained with BAAA and the multidrug-resistance inhibitor verapamil. The SSC(lo)ALDH(br) population was depleted of lineage-committed cells, 40-90% pure for CD34(+)CD38(lo/-) cells, and enriched 50- to 100-fold for primitive hematopoietic progenitors detected in short- and long-term culture analyses. Together, these observations indicate that fractionating human hematopoietic stem cells on the basis of ALDH activity using BAAA is an effective method for isolating primitive human hematopoietic progenitors. This technique may be useful for isolating stem cells from other tissues as well.

Full Text

Cited Authors

  • Storms, RW; Trujillo, AP; Springer, JB; Shah, L; Colvin, OM; Ludeman, SM; Smith, C

Published Date

  • August 3, 1999

Published In

Volume / Issue

  • 96 / 16

Start / End Page

  • 9118 - 9123

PubMed ID

  • 10430905

Pubmed Central ID

  • 10430905

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.96.16.9118

Language

  • eng

Conference Location

  • United States