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CDKN2 in HPV-positive and HPV-negative cervical-carcinoma cell lines.

Publication ,  Journal Article
Kelley, MJ; Otterson, GA; Kaye, FJ; Popescu, NC; Johnson, BE; Dipaolo, JA
Published in: Int J Cancer
October 9, 1995

Human cervical cancers frequently contain retinoblastoma protein (Rb) that is inactivated by binding with human papilloma virus (HPV) E7 protein or through mutation. The CDKN2 gene encodes p16INK4 which inhibits cdk4-cyclin D phosphorylation of Rb, preventing the G1-S transition. To determine whether abnormalities of CDKN2 occur in cervical-cancer cells, II cervical cell lines, including 8 HPV-positive cell lines, 2 HPV-negative cell lines containing mutant Rb, and one tumorigenic cell line derived from normal cervical cells following transfection with HPV-16 and v-H-ras (CX16-2HR), were analyzed. No cell line had a homozygous deletion of exon 1 or 2 of CDKN2, and only one cell line, CX16-2HR, had an altered DNA sequence, which represents a common polymorphism at codon 148. To exclude the possibility of other subtle inactivating mutations, immunoblot analysis of protein lysates was performed using a polyclonal anti-p16INK4 rabbit anti-serum. Abundant levels of normal-sized p16INK4 were observed in all cell samples. Thus, no alterations of CDKN2 were detected in these cervical cell lines. These results confirm that mutational inactivation of p16INK4 is a rare event in tumor samples with compromised Rb activity.

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Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

October 9, 1995

Volume

63

Issue

2

Start / End Page

226 / 230

Location

United States

Related Subject Headings

  • Uterine Cervical Neoplasms
  • Tumor Cells, Cultured
  • Retinoblastoma Protein
  • RNA, Neoplasm
  • RNA, Messenger
  • Polymorphism, Single-Stranded Conformational
  • Point Mutation
  • Papillomaviridae
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
 

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Kelley, M. J., Otterson, G. A., Kaye, F. J., Popescu, N. C., Johnson, B. E., & Dipaolo, J. A. (1995). CDKN2 in HPV-positive and HPV-negative cervical-carcinoma cell lines. Int J Cancer, 63(2), 226–230. https://doi.org/10.1002/ijc.2910630214
Kelley, M. J., G. A. Otterson, F. J. Kaye, N. C. Popescu, B. E. Johnson, and J. A. Dipaolo. “CDKN2 in HPV-positive and HPV-negative cervical-carcinoma cell lines.Int J Cancer 63, no. 2 (October 9, 1995): 226–30. https://doi.org/10.1002/ijc.2910630214.
Kelley MJ, Otterson GA, Kaye FJ, Popescu NC, Johnson BE, Dipaolo JA. CDKN2 in HPV-positive and HPV-negative cervical-carcinoma cell lines. Int J Cancer. 1995 Oct 9;63(2):226–30.
Kelley, M. J., et al. “CDKN2 in HPV-positive and HPV-negative cervical-carcinoma cell lines.Int J Cancer, vol. 63, no. 2, Oct. 1995, pp. 226–30. Pubmed, doi:10.1002/ijc.2910630214.
Kelley MJ, Otterson GA, Kaye FJ, Popescu NC, Johnson BE, Dipaolo JA. CDKN2 in HPV-positive and HPV-negative cervical-carcinoma cell lines. Int J Cancer. 1995 Oct 9;63(2):226–230.
Journal cover image

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

October 9, 1995

Volume

63

Issue

2

Start / End Page

226 / 230

Location

United States

Related Subject Headings

  • Uterine Cervical Neoplasms
  • Tumor Cells, Cultured
  • Retinoblastoma Protein
  • RNA, Neoplasm
  • RNA, Messenger
  • Polymorphism, Single-Stranded Conformational
  • Point Mutation
  • Papillomaviridae
  • Oncology & Carcinogenesis
  • Molecular Sequence Data