The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro.

Journal Article

Virulent, wild-type equine infectious anemia virus (EIAV) is restricted in one or more early steps in replication in equine skin fibroblast cells compared with cell culture-adapted virus, which is fully competent for replication in this cell type. We compared the sequences of wild-type EIAV and a full-length infectious proviral clone of the cell culture-adapted EIAV and found that the genomes were relatively well conserved with the exception of the envelope gene region, which showed extensive sequence differences. We therefore constructed several wild-type and cell culture-adapted virus chimeras to examine the role of the envelope gene in replication in different cell types in vitro. Unlike wild-type virus, which is restricted by an early event(s) for replication in equine dermis cells, the wild-type outer envelope gene chimeras are replication competent in this cell type. We conclude that even though there are extensive sequence differences between wild-type and cell culture-adapted viruses in the surface envelope gene region, this domain is not a determinant of the differing in vitro cell tropisms.

Full Text

Duke Authors

Cited Authors

  • Perry, ST; Flaherty, MT; Kelley, MJ; Clabough, DL; Tronick, SR; Coggins, L; Whetter, L; Lengel, CR; Fuller, F

Published Date

  • July 1992

Published In

Volume / Issue

  • 66 / 7

Start / End Page

  • 4085 - 4097

PubMed ID

  • 1318398

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States