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Small cell lung carcinoma cell lines express mRNA for calcitonin and alpha- and beta-calcitonin gene related peptides.

Publication ,  Journal Article
Kelley, MJ; Snider, RH; Becker, KL; Johnson, BE
Published in: Cancer Lett
June 15, 1994

Calcitonin (CT) and calcitonin gene related peptide (CGRP) are derived from preprohormones encoded by three mRNAs (CT, alpha-CGRP and beta-CGRP) from two genes (CALC1 and CALC2) on chromosome 11. Among 16 small cell lung cancer cell lines examined by RNase protection assay, 9 (56%) had detectable CT mRNA, 8 (50%) had alpha-CGRP mRNA, and 13 (81%) had beta-CGRP mRNA. At least one CALC1 transcript (CT or alpha-CGRP) was found in 11 (69%) cell lines with three having only CT mRNA, two having only alpha-CGRP mRNA, and six having both. beta-CGRP mRNA was detected in all of these 11 cell lines expressing a CALC1 transcript. Immunoreactive CT was detected by radioimmunoassay in eight of nine SCLC cell lines expressing CT mRNA, and immunoreactive CGRP was detected in 12 of 13 cell lines expressing a CGRP mRNA. The variety of expression of these three peptides in different cell lines of the same cell type should provide a useful system for further study of the control of expression of these peptides.

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Published In

Cancer Lett

DOI

ISSN

0304-3835

Publication Date

June 15, 1994

Volume

81

Issue

1

Start / End Page

19 / 25

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Oncology & Carcinogenesis
  • Lung Neoplasms
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Carcinoma, Small Cell
  • Calcitonin Gene-Related Peptide
  • Calcitonin
  • 3211 Oncology and carcinogenesis
 

Citation

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Kelley, M. J., Snider, R. H., Becker, K. L., & Johnson, B. E. (1994). Small cell lung carcinoma cell lines express mRNA for calcitonin and alpha- and beta-calcitonin gene related peptides. Cancer Lett, 81(1), 19–25. https://doi.org/10.1016/0304-3835(94)90159-7
Kelley, M. J., R. H. Snider, K. L. Becker, and B. E. Johnson. “Small cell lung carcinoma cell lines express mRNA for calcitonin and alpha- and beta-calcitonin gene related peptides.Cancer Lett 81, no. 1 (June 15, 1994): 19–25. https://doi.org/10.1016/0304-3835(94)90159-7.
Kelley MJ, Snider RH, Becker KL, Johnson BE. Small cell lung carcinoma cell lines express mRNA for calcitonin and alpha- and beta-calcitonin gene related peptides. Cancer Lett. 1994 Jun 15;81(1):19–25.
Kelley, M. J., et al. “Small cell lung carcinoma cell lines express mRNA for calcitonin and alpha- and beta-calcitonin gene related peptides.Cancer Lett, vol. 81, no. 1, June 1994, pp. 19–25. Pubmed, doi:10.1016/0304-3835(94)90159-7.
Kelley MJ, Snider RH, Becker KL, Johnson BE. Small cell lung carcinoma cell lines express mRNA for calcitonin and alpha- and beta-calcitonin gene related peptides. Cancer Lett. 1994 Jun 15;81(1):19–25.
Journal cover image

Published In

Cancer Lett

DOI

ISSN

0304-3835

Publication Date

June 15, 1994

Volume

81

Issue

1

Start / End Page

19 / 25

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Oncology & Carcinogenesis
  • Lung Neoplasms
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Carcinoma, Small Cell
  • Calcitonin Gene-Related Peptide
  • Calcitonin
  • 3211 Oncology and carcinogenesis