Mutational analysis of the tyrosine phosphatome in colorectal cancers.

Published

Journal Article

Tyrosine phosphorylation, regulated by protein tyrosine phosphatases (PTPs) and kinases (PTKs), is important in signaling pathways underlying tumorigenesis. A mutational analysis of the tyrosine phosphatase gene superfamily in human cancers identified 83 somatic mutations in six PTPs (PTPRF, PTPRG, PTPRT, PTPN3, PTPN13, PTPN14), affecting 26% of colorectal cancers and a smaller fraction of lung, breast, and gastric cancers. Fifteen mutations were nonsense, frameshift, or splice-site alterations predicted to result in truncated proteins lacking phosphatase activity. Five missense mutations in the most commonly altered PTP (PTPRT) were biochemically examined and found to reduce phosphatase activity. Expression of wild-type but not a mutant PTPRT in human cancer cells inhibited cell growth. These observations suggest that the mutated tyrosine phosphatases are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention.

Full Text

Duke Authors

Cited Authors

  • Wang, Z; Shen, D; Parsons, DW; Bardelli, A; Sager, J; Szabo, S; Ptak, J; Silliman, N; Peters, BA; van der Heijden, MS; Parmigiani, G; Yan, H; Wang, T-L; Riggins, G; Powell, SM; Willson, JKV; Markowitz, S; Kinzler, KW; Vogelstein, B; Velculescu, VE

Published Date

  • May 21, 2004

Published In

Volume / Issue

  • 304 / 5674

Start / End Page

  • 1164 - 1166

PubMed ID

  • 15155950

Pubmed Central ID

  • 15155950

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1096096

Language

  • eng

Conference Location

  • United States