Quantitative microchemical imaging of calcium in Na-K pump inhibited heart cells.

Journal Article (Journal Article)

Quantitative electron probe X-ray imaging techniques have been utilized to determine simultaneously the element content within a single cultured embryonic chick heart cell and its intracellular compartments as well as the average elemental content of several heart cells within a population. These features of microchemical imaging have permitted establishment of data regarding: (1) the heterogeneity of calcium accumulation in mitochondrial, cytoplasmic and nuclear compartments under conditions which elevate total cell calcium without producing irreversible cell injury; and (2) the variability of calcium accumulation from cell to cell within the population sampled. The results indicate that during Na-K pump inhibition (K-free HT-BSS, 10(-4) M ouabain, 60 min) elevation of mitochondrial calcium, measured in situ by electron probe X-ray microanalysis, to levels more than 100 times greater than in the basal state, may not cause irreversible mitochondrial uncoupling and cell death.

Full Text

Duke Authors

Cited Authors

  • LeFurgey, A; Ingram, P; Lieberman, M

Published Date

  • December 1988

Published In

Volume / Issue

  • 9 / 5-6

Start / End Page

  • 219 - 235

PubMed ID

  • 2852062

International Standard Serial Number (ISSN)

  • 0143-4160

Digital Object Identifier (DOI)

  • 10.1016/0143-4160(88)90003-6


  • eng

Conference Location

  • Netherlands