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Suspension of medullary thick ascending limb tubules from the rabbit kidney.

Publication ,  Journal Article
Chamberlin, ME; LeFurgey, A; Mandel, LJ
Published in: Am J Physiol
December 1984

A procedure for isolating a suspension of tubules derived from the rabbit medullary thick ascending limb is described. The purity of the preparation was assessed by microscopy and enzyme assays and the viability of the preparation was assessed by measuring oxygen consumption. Microscopy revealed that the suspension contains 95% thick ascending limbs and that the isolation procedure preserves the structure of the epithelium except for the loss of the basement membrane. The preparation had a high activity of calcitonin-sensitive adenylate cyclase, a marker enzyme for the medullary thick ascending limb. Control oxygen consumption was considerably higher than that reported for proximal tubules in the literature, and nystatin or carbonyl cyanide p-trifluoromethoxyphenylhydrazone addition produced a more than 100% increase in oxygen consumption. Furosemide inhibited the oxygen consumption by 43% and ouabain inhibited it by 42%. Furosemide inhibited sodium chloride entry without directly affecting the Na-K-ATPase or cellular metabolism. Chloride removal depressed oxygen consumption to the same extent as furosemide, but some of this action was through direct inhibition of cellular metabolism.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1984

Volume

247

Issue

6 Pt 2

Start / End Page

F955 / F964

Location

United States

Related Subject Headings

  • Sodium-Potassium-Exchanging ATPase
  • Rabbits
  • Potassium
  • Oxygen Consumption
  • Ouabain
  • Loop of Henle
  • Kidney Tubules
  • Kidney Medulla
  • Histological Techniques
  • Furosemide
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chamberlin, M. E., LeFurgey, A., & Mandel, L. J. (1984). Suspension of medullary thick ascending limb tubules from the rabbit kidney. Am J Physiol, 247(6 Pt 2), F955–F964. https://doi.org/10.1152/ajprenal.1984.247.6.F955
Chamberlin, M. E., A. LeFurgey, and L. J. Mandel. “Suspension of medullary thick ascending limb tubules from the rabbit kidney.Am J Physiol 247, no. 6 Pt 2 (December 1984): F955–64. https://doi.org/10.1152/ajprenal.1984.247.6.F955.
Chamberlin ME, LeFurgey A, Mandel LJ. Suspension of medullary thick ascending limb tubules from the rabbit kidney. Am J Physiol. 1984 Dec;247(6 Pt 2):F955–64.
Chamberlin, M. E., et al. “Suspension of medullary thick ascending limb tubules from the rabbit kidney.Am J Physiol, vol. 247, no. 6 Pt 2, Dec. 1984, pp. F955–64. Pubmed, doi:10.1152/ajprenal.1984.247.6.F955.
Chamberlin ME, LeFurgey A, Mandel LJ. Suspension of medullary thick ascending limb tubules from the rabbit kidney. Am J Physiol. 1984 Dec;247(6 Pt 2):F955–F964.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1984

Volume

247

Issue

6 Pt 2

Start / End Page

F955 / F964

Location

United States

Related Subject Headings

  • Sodium-Potassium-Exchanging ATPase
  • Rabbits
  • Potassium
  • Oxygen Consumption
  • Ouabain
  • Loop of Henle
  • Kidney Tubules
  • Kidney Medulla
  • Histological Techniques
  • Furosemide