Effects of colchicine and cytochalasin B on hypertonicity-induced changes in toad urinary bladder.

Journal Article (Journal Article)

Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initiated to establish whether the transformation is mediated by the cytoskeletal network and, thus, can be prevented by disruption of microtubule-microfilament function with colchicine or cytochalasin B (CB). Scanning electron microscopy revealed the characteristic branching ridges on granular cells of control bladder incubated with colchicine or CB. In contrast, transformation of ridges to discrete microvilli was observed in experimental bladders exposed to serosal hypertonicity alone or in combination with either colchicine or CB. These results suggest that the mechanism underlying hypertonicity-induced surface changes which are associated with increased water permeability does not involve either microtubules or microfilaments.

Full Text

Duke Authors

Cited Authors

  • Dratwa, M; LeFurgey, A; Tisher, CC

Published Date

  • 1984

Published In

Volume / Issue

  • 236 / 3

Start / End Page

  • 585 - 591

PubMed ID

  • 6432330

International Standard Serial Number (ISSN)

  • 0302-766X

Digital Object Identifier (DOI)

  • 10.1007/BF00217226


  • eng

Conference Location

  • Germany