Upstream stimulatory factors are mediators of Ca2+-responsive transcription in neurons.

Published

Journal Article

To identify molecular mechanisms that control activity-dependent gene expression in the CNS, we have characterized the factors that mediate activity-dependent transcription of BDNF promoter III. We report the identification of a Ca(2+)-responsive E-box element, CaRE2, within BDNF promoter III that binds upstream stimulatory factors 1 and 2 (USF1/2) and show that USFs are required for the activation of CaRE2-dependent transcription from BDNF promoter III. We find that the transcriptional activity of the USFs is regulated by Ca(2+)-activated signaling pathways in neurons and that the USFs bind to the promoters of a number of neuronal activity-regulated genes in vivo. These results suggest a new function for the USFs in the regulation of activity-dependent transcription in neurons.

Full Text

Duke Authors

Cited Authors

  • Chen, WG; West, AE; Tao, X; Corfas, G; Szentirmay, MN; Sawadogo, M; Vinson, C; Greenberg, ME

Published Date

  • April 1, 2003

Published In

Volume / Issue

  • 23 / 7

Start / End Page

  • 2572 - 2581

PubMed ID

  • 12684442

Pubmed Central ID

  • 12684442

Electronic International Standard Serial Number (EISSN)

  • 1529-2401

Language

  • eng

Conference Location

  • United States