Preclinical evaluation and PET imaging of 18F-labeled Mel-14 F(ab')2 fragment in normal dogs.
Journal Article (Journal Article)
The F(ab')2 fragment of monoclonal antibody Mel-14, reactive with human melanomas and gliomas, was labeled with 18F using two acylation agents, N-succinimidyl 8-[(4'-[18F]fluorobenzyl)amino]suberate (SFBS) and N-succinimidyl 4-[18F]fluorobenzoate (SFB). The immunoreactivity and affinity for Mel-14 F(ab')2 labeled using the two methods were similar. As a prelude to human clinical evaluation, PET imaging, tissue distribution and pharmacokinetic measurements were performed in two groups of normal foxhounds. Similar in vivo behavior was seen for Mel-14 F(ab')2 labeled using SFBS and SFB. Radiation dosimetry calculations suggest that a 10 mCi dose could be used for this F(ab')2 fragment labeled using either acylation agent.
Full Text
Duke Authors
Cited Authors
- Page, RL; Garg, PK; Vaidyanathan, G; Zalutsky, MR
Published Date
- October 1994
Published In
Volume / Issue
- 21 / 7
Start / End Page
- 911 - 919
PubMed ID
- 9234344
International Standard Serial Number (ISSN)
- 0969-8051
Digital Object Identifier (DOI)
- 10.1016/0969-8051(94)90079-5
Language
- eng
Conference Location
- United States