The Ras-related protein AGS1/RASD1 suppresses cell growth.

Published

Journal Article

AGS1/RASD1 is a Ras-related protein identified as a dexamethasone-inducible cDNA and as a signal regulator in various functional and protein-interaction screens. As an initial approach to define the role of AGS1/RASD1 as a Ras-family member, we determined its influence on cell growth/survival. In clonogenic assays with NIH-3T3 murine fibroblast cells, the MCF-7 human breast cancer cell line and the human lung adenocarcinoma cell line A549, AGS1/RASD1 markedly diminished the number of G418-resistant colonies, whereas the Ras subgroup member K-Ras was without effect. A549 cell infection with adenovirus engineered to express AGS1/RASD1 (Ad.AGS1) inhibited log phase growth in vitro and increased the percentage of cells undergoing apoptosis. The anti-growth action was also observed in vivo as the expression of AGS1/RASD1 inhibited the subcutaneous tumor growth of A549 cells in athymic nude mice. These data indicate that AGS1/RASD1, a member of the Ras superfamily of small G-proteins that often promotes cell growth and tumor expansion, plays an active role in preventing aberrant cell growth.

Full Text

Duke Authors

Cited Authors

  • Vaidyanathan, G; Cismowski, MJ; Wang, G; Vincent, TS; Brown, KD; Lanier, SM

Published Date

  • July 29, 2004

Published In

Volume / Issue

  • 23 / 34

Start / End Page

  • 5858 - 5863

PubMed ID

  • 15184869

Pubmed Central ID

  • 15184869

International Standard Serial Number (ISSN)

  • 0950-9232

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1207774

Language

  • eng

Conference Location

  • England