Magnetic resonance histology for morphologic phenotyping.

Published

Journal Article

Magnetic resonance histology (MRH) images of the whole mouse have been acquired at 100-micron isotropic resolution at 2.0 T with image arrays of 256 x 256 x 1024. Higher resolution (50 x 50 x 50 microns) of limited volumes has been acquired at 7.1T with image arrays of 512 x 512 x 512. Even higher resolution images (20 x 20 x 20 microns) of isolated organs have been acquired at 9.4 T. The volume resolution represents an increase of 625000 x over conventional clinical MRI. The technological basis is summarized that will allow basic scientists to begin using MRH as a routine method for morphologcic phenotyping of the mouse. MRH promises four unique attributes over conventional histology: 1). MRH is non-destructive; 2). MRH exploits the unique contrast mechanisms that have made MRI so successful clinically; 3). MRH is 3-dimensional; and 4). the data are inherently digital. We demonstrate the utility in morphologic phenotyping a whole C57BL/6J mouse.

Full Text

Duke Authors

Cited Authors

  • Johnson, GA; Cofer, GP; Fubara, B; Gewalt, SL; Hedlund, LW; Maronpot, RR

Published Date

  • October 2002

Published In

Volume / Issue

  • 16 / 4

Start / End Page

  • 423 - 429

PubMed ID

  • 12353257

Pubmed Central ID

  • 12353257

International Standard Serial Number (ISSN)

  • 1053-1807

Digital Object Identifier (DOI)

  • 10.1002/jmri.10175

Language

  • eng

Conference Location

  • United States