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Mechanism of detection of acute cerebral ischemia in rats by diffusion-weighted magnetic resonance microscopy.

Publication ,  Journal Article
Benveniste, H; Hedlund, LW; Johnson, GA
Published in: Stroke
May 1992

BACKGROUND AND PURPOSE: The aim of this study was to measure apparent diffusion coefficients in rat brain tissue exposed to ouabain, glutamate, and N-methyl-D-aspartate and to compare them with apparent diffusion coefficients found in acute cerebral ischemia. METHODS: The apparent diffusion coefficient was measured using magnetic resonance microscopy in four groups of Sprague-Dawley rats after occlusion of the right middle cerebral artery and ipsilateral common carotid artery (n = 7), after ouabain exposure (n = 6), during glutamate exposure (n = 7), or during N-methyl-D-aspartate exposure (n = 3). Ouabain, glutamate, and N-methyl-D-aspartate were applied via an intracerebrally implanted microdialysis membrane. RESULTS: Three hours after the induction of focal cerebral ischemia, a 33% reduction in the apparent diffusion coefficient was observed in the right dorsolateral corpus striatum and olfactory cortex. After ouabain exposure, reductions in the apparent diffusion coefficient were observed within a 1,500-microns radius of the microdialysis membrane. Quantitative analysis revealed that apparent diffusion coefficient values in ischemic and ouabain-exposed tissue fell within the same range. Glutamate and N-methyl-D-aspartate reduced the brain tissue apparent diffusion coefficient by 35% and 40%, respectively. CONCLUSIONS: On the basis of these findings, we conclude that ischemia-induced apparent diffusion coefficient reductions are likely caused by a shift of extracellular to intracellular water.

Duke Scholars

Published In

Stroke

DOI

ISSN

0039-2499

Publication Date

May 1992

Volume

23

Issue

5

Start / End Page

746 / 754

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Rats, Inbred Strains
  • Rats
  • Ouabain
  • Neurology & Neurosurgery
  • N-Methylaspartate
  • Magnetic Resonance Imaging
  • Glutamic Acid
  • Glutamates
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Benveniste, H., Hedlund, L. W., & Johnson, G. A. (1992). Mechanism of detection of acute cerebral ischemia in rats by diffusion-weighted magnetic resonance microscopy. Stroke, 23(5), 746–754. https://doi.org/10.1161/01.str.23.5.746
Benveniste, H., L. W. Hedlund, and G. A. Johnson. “Mechanism of detection of acute cerebral ischemia in rats by diffusion-weighted magnetic resonance microscopy.Stroke 23, no. 5 (May 1992): 746–54. https://doi.org/10.1161/01.str.23.5.746.
Benveniste, H., et al. “Mechanism of detection of acute cerebral ischemia in rats by diffusion-weighted magnetic resonance microscopy.Stroke, vol. 23, no. 5, May 1992, pp. 746–54. Pubmed, doi:10.1161/01.str.23.5.746.

Published In

Stroke

DOI

ISSN

0039-2499

Publication Date

May 1992

Volume

23

Issue

5

Start / End Page

746 / 754

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Rats, Inbred Strains
  • Rats
  • Ouabain
  • Neurology & Neurosurgery
  • N-Methylaspartate
  • Magnetic Resonance Imaging
  • Glutamic Acid
  • Glutamates
  • Female