Magnetic resonance microscopy of embryos.
Journal Article (Journal Article)
We demonstrate that magnetic resonance (MR) microscopy provides a mechanism to investigate normal and abnormal developmental anatomy in a non-destructive and distortion-free manner. Techniques for the fixation, embedding, perfusion and image acquisition of embryos between 3 and 30 mm crown rump length are described. We describe the perfusion of a contrast agent to enhance images of the developing embryonic vasculature. Data are acquired as three-dimensional isotropic arrays which permit images to be reformatted retrospectively in any plane. The data are available for archiving, distributing and for post-acquisition manipulations. MR microscopy is a fast technique for producing three-dimensional reconstructions and is free from registration and sectioning artifacts.
Full Text
Duke Authors
Cited Authors
- Smith, BR; Linney, E; Huff, DS; Johnson, GA
Published Date
- 1996
Published In
Volume / Issue
- 20 / 6
Start / End Page
- 483 - 490
PubMed ID
- 9007215
International Standard Serial Number (ISSN)
- 0895-6111
Digital Object Identifier (DOI)
- 10.1016/s0895-6111(96)00046-8
Language
- eng
Conference Location
- United States