Imaging inflammation: direct visualization of perivascular cuffing in EAE by magnetic resonance microscopy.

Published

Journal Article

PURPOSE: To determine if the architectural features revealed by magnetic resonance microscopy (MRM) allow one to detect microscopic abnormalities associated with neuroinflammation in fixed brain sections from animals with experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS). MATERIALS AND METHODS: Imaging was performed at the Center for In Vivo Microscopy (CIVM) using a 9.4-Tesla, 89-mm bore, superconducting magnet with actively shielded gradients capable of 850 mT/m. A number of MR contrasts and spatial resolutions were explored. RESULTS: The assessment of EAE brain showed that it is possible to visualize perivascular cuffing in vitro by MRM on three-dimensional T1 proton stains. CONCLUSION: Inflammatory cell infiltration is a prerequisite for the development of lesions in EAE and MS. Thus, the ability to directly detect individual perivascular cuffs of inflammation may provide a useful means of monitoring the time course of inflammatory events, as conventional histopathological scoring of perivascular cuffs is utilized, but in the absence of sectioning and staining.

Full Text

Duke Authors

Cited Authors

  • Gareau, PJ; Wymore, AC; Cofer, GP; Johnson, GA

Published Date

  • July 2002

Published In

Volume / Issue

  • 16 / 1

Start / End Page

  • 28 - 36

PubMed ID

  • 12112500

Pubmed Central ID

  • 12112500

International Standard Serial Number (ISSN)

  • 1053-1807

Digital Object Identifier (DOI)

  • 10.1002/jmri.10136

Language

  • eng

Conference Location

  • United States