Improved preparation of chick embryonic samples for magnetic resonance microscopy.


Journal Article

Previous work demonstrated the power of three-dimensional (3D) magnetic resonance microscopy (MRM) to follow complicated morphologic development in the embryonic cardiovascular system. In this study we describe a new dual-contrast method for specimen preparation that combines perfusion fixation and immersion in fixative with macro- and small molecular gadolinium agents to provide enhanced definition of both the heart wall and chamber. MRM was performed at 9.4 T with image resolutions of 25, 31, and 50 microm isotropic voxels for three stages of chick embryos (day 4, day 5.5, and day 9), and compared to histological sections of the same embryos. The results show considerable improvement of image quality over previous efforts, with better signal-to-noise ratio (SNR) and contrast between the cardiac chamber and myocardial wall. Excellent correlation was shown between the MRM images and histological sections. Thus, 3D high-resolution MRM in combination with the dual-contrast technique is useful for acquiring quantitative 3D morphologic data regarding heart development.

Full Text

Duke Authors

Cited Authors

  • Zhang, X; Yelbuz, TM; Cofer, GP; Choma, MA; Kirby, ML; Johnson, GA

Published Date

  • June 2003

Published In

Volume / Issue

  • 49 / 6

Start / End Page

  • 1192 - 1195

PubMed ID

  • 12768599

Pubmed Central ID

  • 12768599

International Standard Serial Number (ISSN)

  • 0740-3194

Digital Object Identifier (DOI)

  • 10.1002/mrm.10460


  • eng

Conference Location

  • United States