Skip to main content
Journal cover image

Time-course imaging of rat embryos in utero with magnetic resonance microscopy.

Publication ,  Journal Article
Smith, BR; Shattuck, MD; Hedlund, LW; Johnson, GA
Published in: Magn Reson Med
April 1998

Magnetic resonance (MR) microscopy was used to noninvasively investigate the development of live rat embryos in utero. The difficulty in making sequential observations of a developing mammalian embryo has frustrated developmental biologists for many years. Most current technologies analyze normal and abnormal development by observing end point phenotypes (in fixed specimens) rather than investigating the live embryo. MR microscopy was adapted to allow rat litters to be scanned three times each (at 1- to 3-day intervals) and has produced images of live developing embryos. It was demonstrated that repeated anesthesia and imaging protocols produced no gross malformations in the rat pups that were subsequently delivered and observed. Three-dimensional projection encoding with phase rewinders produced isotropic [256(3)] image data sets in about 30 minutes with excellent tissue contrast arising from steady-state effects in the amniotic fluid.

Duke Scholars

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

April 1998

Volume

39

Issue

4

Start / End Page

673 / 677

Location

United States

Related Subject Headings

  • Uterus
  • Time Factors
  • Rats, Sprague-Dawley
  • Rats
  • Pregnancy
  • Nuclear Medicine & Medical Imaging
  • Magnetic Resonance Imaging
  • Image Processing, Computer-Assisted
  • Gestational Age
  • Follow-Up Studies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Smith, B. R., Shattuck, M. D., Hedlund, L. W., & Johnson, G. A. (1998). Time-course imaging of rat embryos in utero with magnetic resonance microscopy. Magn Reson Med, 39(4), 673–677. https://doi.org/10.1002/mrm.1910390424
Smith, B. R., M. D. Shattuck, L. W. Hedlund, and G. A. Johnson. “Time-course imaging of rat embryos in utero with magnetic resonance microscopy.Magn Reson Med 39, no. 4 (April 1998): 673–77. https://doi.org/10.1002/mrm.1910390424.
Smith BR, Shattuck MD, Hedlund LW, Johnson GA. Time-course imaging of rat embryos in utero with magnetic resonance microscopy. Magn Reson Med. 1998 Apr;39(4):673–7.
Smith, B. R., et al. “Time-course imaging of rat embryos in utero with magnetic resonance microscopy.Magn Reson Med, vol. 39, no. 4, Apr. 1998, pp. 673–77. Pubmed, doi:10.1002/mrm.1910390424.
Smith BR, Shattuck MD, Hedlund LW, Johnson GA. Time-course imaging of rat embryos in utero with magnetic resonance microscopy. Magn Reson Med. 1998 Apr;39(4):673–677.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

April 1998

Volume

39

Issue

4

Start / End Page

673 / 677

Location

United States

Related Subject Headings

  • Uterus
  • Time Factors
  • Rats, Sprague-Dawley
  • Rats
  • Pregnancy
  • Nuclear Medicine & Medical Imaging
  • Magnetic Resonance Imaging
  • Image Processing, Computer-Assisted
  • Gestational Age
  • Follow-Up Studies