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Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice.

Publication ,  Journal Article
Chen, BT; Yordanov, AT; Johnson, GA
Published in: Magn Reson Med
January 2005

Increasing use of transgenic animal models for pulmonary disease has raised the need for methods to assess pulmonary structure and function in a physiologically stable mouse. We report here an integrated protocol using magnetic resonance microscopy with gadolinium (Gd)-labeled starburst dendrimer (G6-1B4M-Gd, MW = 192 +/- 1 kDa, R(h) = 5.50 +/- 0.04 nm) and hyperpolarized (3)helium ((3)He) gas to acquire images that demonstrate pulmonary vasculature and ventilated airways in live mice (n = 9). Registered three-dimensional images of (1)H and (3)He were acquired during breath-hold at 2.0 T using radial acquisition (total acquisition time of 38 and 25 min, respectively). The macromolecular Gd-labeled dendrimer (a half-life of approximately 80 min) increased the signal-to-noise by 81 +/- 30% in the left ventricle, 43 +/- 22% in the lung periphery, and -4 +/- 5% in the chest wall, thus increasing the contrast of these structures relative to the less vascular surrounding tissues. A constant-flow ventilator was developed for the mouse to deliver varied gas mixtures of O(2) and N(2) (or (3)He) during imaging. To avoid hypoxemia, instrumental dead space was minimized and corrections were made to tidal volume lost due to gas compression. The stability of the physiologic support was assessed by the lack of spontaneous breathing and maintenance of a constant heart rate. We were able to stabilize the mouse for >8 hr using ventilation of 105 breath/min and approximately 0.2 mL/breath. The feasibility of acquiring both pulmonary vasculature and ventilated airways was demonstrated in the mouse lung with in-plane spatial resolution of 70 x 70 microm(2) and slice thickness of 800 microm.

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Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

January 2005

Volume

53

Issue

1

Start / End Page

69 / 75

Location

United States

Related Subject Headings

  • Ventilation-Perfusion Ratio
  • Respiration, Artificial
  • Pulmonary Ventilation
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Magnetic Resonance Spectroscopy
  • Magnetic Resonance Imaging
  • Lung
 

Citation

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ICMJE
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Chen, B. T., Yordanov, A. T., & Johnson, G. A. (2005). Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice. Magn Reson Med, 53(1), 69–75. https://doi.org/10.1002/mrm.20307
Chen, Ben T., Alexander T. Yordanov, and G Allan Johnson. “Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice.Magn Reson Med 53, no. 1 (January 2005): 69–75. https://doi.org/10.1002/mrm.20307.
Chen BT, Yordanov AT, Johnson GA. Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice. Magn Reson Med. 2005 Jan;53(1):69–75.
Chen, Ben T., et al. “Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice.Magn Reson Med, vol. 53, no. 1, Jan. 2005, pp. 69–75. Pubmed, doi:10.1002/mrm.20307.
Chen BT, Yordanov AT, Johnson GA. Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice. Magn Reson Med. 2005 Jan;53(1):69–75.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

January 2005

Volume

53

Issue

1

Start / End Page

69 / 75

Location

United States

Related Subject Headings

  • Ventilation-Perfusion Ratio
  • Respiration, Artificial
  • Pulmonary Ventilation
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Magnetic Resonance Spectroscopy
  • Magnetic Resonance Imaging
  • Lung