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Myocardial volume and organization are changed by failure of addition of secondary heart field myocardium to the cardiac outflow tract.

Publication ,  Journal Article
Yelbuz, TM; Waldo, KL; Zhang, X; Zdanowicz, M; Parker, J; Creazzo, TL; Johnson, GA; Kirby, ML
Published in: Dev Dyn
October 2003

Cardiac neural crest ablation results in primary myocardial dysfunction and failure of the secondary heart field to add the definitive myocardium to the cardiac outflow tract. The current study was undertaken to understand the changes in myocardial characteristics in the heart tube, including volume, proliferation, and cell size when the myocardium from the secondary heart field fails to be added to the primary heart tube. We used magnetic resonance and confocal microscopy to determine that the volume of myocardium in the looped heart was dramatically reduced and the compact layer of myocardium was thinner after neural crest ablation, especially in the outflow tract and ventricular regions. Proliferation measured by 5-bromo-2'-deoxyuridine incorporation was elevated at only one stage during looping, cell death was normal and myocardial cell size was increased. Taken together, these results indicate that there are fewer myocytes in the heart. By incubation day 8 when the heart would have normally completed septation, the anterior (ventral) wall of the right ventricle and right ventricular outflow tract was significantly thinner in the neural crest-ablated embryos than normal, but the thickness of the compact myocardium was normal in all other regions of the heart. The decreased volume and number of myocardial cells in the heart tube after neural crest ablation most likely reflects the amount of myocardium added by the secondary heart field.

Duke Scholars

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

October 2003

Volume

228

Issue

2

Start / End Page

152 / 160

Location

United States

Related Subject Headings

  • Time Factors
  • Neural Crest
  • Myocytes, Cardiac
  • Myocardium
  • Microscopy, Confocal
  • Magnetic Resonance Imaging
  • Immunohistochemistry
  • Heart Ventricles
  • Heart Defects, Congenital
  • Heart Atria
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yelbuz, T. M., Waldo, K. L., Zhang, X., Zdanowicz, M., Parker, J., Creazzo, T. L., … Kirby, M. L. (2003). Myocardial volume and organization are changed by failure of addition of secondary heart field myocardium to the cardiac outflow tract. Dev Dyn, 228(2), 152–160. https://doi.org/10.1002/dvdy.10364
Yelbuz, T Mesud, Karen L. Waldo, Xiaowei Zhang, Marzena Zdanowicz, Jeremy Parker, Tony L. Creazzo, G Allan Johnson, and Margaret L. Kirby. “Myocardial volume and organization are changed by failure of addition of secondary heart field myocardium to the cardiac outflow tract.Dev Dyn 228, no. 2 (October 2003): 152–60. https://doi.org/10.1002/dvdy.10364.
Yelbuz TM, Waldo KL, Zhang X, Zdanowicz M, Parker J, Creazzo TL, et al. Myocardial volume and organization are changed by failure of addition of secondary heart field myocardium to the cardiac outflow tract. Dev Dyn. 2003 Oct;228(2):152–60.
Yelbuz, T. Mesud, et al. “Myocardial volume and organization are changed by failure of addition of secondary heart field myocardium to the cardiac outflow tract.Dev Dyn, vol. 228, no. 2, Oct. 2003, pp. 152–60. Pubmed, doi:10.1002/dvdy.10364.
Yelbuz TM, Waldo KL, Zhang X, Zdanowicz M, Parker J, Creazzo TL, Johnson GA, Kirby ML. Myocardial volume and organization are changed by failure of addition of secondary heart field myocardium to the cardiac outflow tract. Dev Dyn. 2003 Oct;228(2):152–160.
Journal cover image

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

October 2003

Volume

228

Issue

2

Start / End Page

152 / 160

Location

United States

Related Subject Headings

  • Time Factors
  • Neural Crest
  • Myocytes, Cardiac
  • Myocardium
  • Microscopy, Confocal
  • Magnetic Resonance Imaging
  • Immunohistochemistry
  • Heart Ventricles
  • Heart Defects, Congenital
  • Heart Atria